Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir

Akbarningrum Fatmawati; Rudy Agustriyanto; Yusnita Liasari

doi:10.9767/bcrec.8.1.4048.34-39

DOI: https://doi.org/10.9767/bcrec.8.1.4048.34-39

Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir

Akbarningrum Fatmawati¹ , Rudy Agustriyanto¹, Yusnita Liasari²

¹Department of Chemical Engineering, Surabaya University, Jl. Raya Kalirungkut, Surabaya 60292, Indonesia

²Biotechnology Faculty, Surabaya University, Jl. Raya Kalirungkut, Surabaya 60292, Indonesia

Received: 2 Oct 2012; Revised: 31 Jan 2013; Accepted: 6 Feb 2013; Available online: 18 Jun 2013; Published: 30 Jun 2013.

Editor(s): Istadi Istadi

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Abstract

The purpose of this research is to study the effect of concentration and temperature on the cellulose and lignin content, and the reducing sugars produced in the enzymatic hydrolysis of coconut coir. In this research, the coconut coir is pretreated using 3%, 7%, and 11% NaOH solution at 60oC, 80oC, and 100oC. The pretreated coir were assayed by measuring the amount of cellulose and lignin and then hydrolysed using Celluclast and Novozyme 188 under various temperature (30oC, 40oC, 50oC) and pH (3, 4, 5). The hydrolysis results were assayed for the reducing sugar content. The results showed that the alkaline delignification was effective to reduce lignin and to increase the cellulose content of the coir. The best delignification condition was observed at 11% NaOH solution and 100oC which removed 14,53% of lignin and increased the cellulose content up to 50,23%. The best condition of the enzymatic hydrolysis was obtained at 50oC and pH 4 which produced 7,57 gr/L reducing sugar. © 2013 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0)

Keywords: coconut; enzyme; hydrolysis; lignocellulose

Funding: Surabaya University

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Section: Original Research Articles

Language : EN

In 2013: BCREC Volume 8 Issue 1 Year 2013 (June 2013)

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