Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent

Sari Edi Cahyaningrum; Maria Monica Sianita

doi:10.9767/bcrec.9.3.7060.263-269

DOI: https://doi.org/10.9767/bcrec.9.3.7060.263-269

Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent

Sari Edi Cahyaningrum , Maria Monica Sianita

Department of Chemistry, Faculty Mathematics and Natural Science, State University of Surabaya, Jl. Ketintang Surabaya 60231, Indonesia

Received: 1 Jul 2014; Revised: 3 Sep 2014; Accepted: 3 Sep 2014; Available online: 27 Oct 2014; Published: 30 Dec 2014.

Editor(s): Istadi Istadi

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Abstract

Glutaraldehyde crosslinked chitosan - silica nanobeads have been used as a support in the immobiliza-tion process of pepsin. The effect of pH, pepsin concentration, and temperature on the characteristics of both free and immobilized pepsin had been investigated in this study. The results showed that the im-mobilized pepsin has wider pH range, better capacity, performance and stability to high temperatures compared with those of free pepsin. The immobilized pepsin can be used for 8 to 10 times at which the activity remains 20%, whereas the free pepsin can only be used once. These results suggested promis-ing applications of immobilized enzyme as biocatalyst. © 2014 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0)

Keywords: immobilized pepsin; chitosan; silica; glutaraldehyde crosslinker

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Section: Original Research Articles

Language : EN

In 2014: BCREC Volume 9 Issue 3 Year 2014 (December 2014)

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