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Green Synthesis of ZnO Nanoparticles using Aloe Vera Extract and Xanthan Gum as Modifier for Photocatalytic Degradation of Anionic and Cationic Dye in Aqueous Solution

Departement of Chemistry, UIN Sunan Kalijaga, Yogyakarta 55282, Indonesia

Received: 24 Sep 2025; Revised: 2 Dec 2025; Accepted: 3 Dec 2025; Available online: 20 Dec 2025; Published: 30 Apr 2026.
Editor(s): Istadi Istadi
Open Access Copyright (c) 2026 by Authors, Published by BCREC Publishing Group
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
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Abstract

The green synthesis of Zinc Oxide (ZnO) nanoparticles is a simpler, low-energy method that avoids toxic chemicals, making the process more cost-effective and environmentally friendly. The green synthesis was performed using aloe vera extract (55% - Aloin), rich in electrons from its hydroxyl groups, as a reducing agent, and natural polysaccharides from xanthan gum to disperse particles and prevent agglomeration. The green synthesis product was characterized using scanning electron microscopy, Fourier Transform spectroscopy, X-ray diffraction, transmission electron microscopy, and Diffuse Reflectance UV spectroscopy. The green-synthesized ZnO nanoparticles, both with xanthan gum (ZnO-AL/XG) and without xanthan gum (ZnO-AL), adopted a hexagonal wurtzite crystal structure. The addition of xanthan gum significantly reduced the crystallite size and enhanced the surface homogeneity of the photocatalyst. Over 50% removal of both anionic and cationic dyes was achieved by ZnO-AL/XG for up to 3 uses, and by ZnO-AL for up to 2 uses, respectively. These findings highlight the potential of the aloe vera–xanthan gum-based green synthesis as a sustainable and efficient strategy for producing ZnO nanomaterials applicable in dye wastewater treatment. Copyright © 2026 by Authors, Published by BCREC Publishing Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Keywords: Green synthesis; ZnO nanoparticles; Aloe vera extract; Xanthan gum; Photocatalytic degradation
Funding: Sunan Kalijaga State Islamic University

Article Metrics:

  1. Hassani, A., Pourshirband, N., Sayyar, Z., Eghbali, P. (2025). Fenton and Fenton-like-based advanced oxidation processes. In: Innovative and Hybrid Advanced Oxidation Processes for Water Treatment. 1st Edition - November 5, 2024, 171–203. DOI: 10.1016/B978-0-443-14100-3.00006-5
  2. Lama, G., Meijide, J., Sanromán, A., Pazos, M. (2022). Heterogeneous Advanced Oxidation Processes: Current Approaches for Wastewater Treatment. Catalysts, 12(3), 344. DOI: 10.3390/catal12030344
  3. Swain, M., Mishra, D. & Sahoo, G. A (2025) review on green synthesis of ZnO nanoparticles. Discov. Appl. Sci. 7, 997. DOI: 10.1007/s42452-025-06957-8
  4. Nandhin, J., Karthikeyan, E., Rajeshkumar, S. (2024). Green synthesis of zinc oxide nanoparticles: Eco-friendly advancements for biomedical marvels. Resources Chemicals and Materials, 3(4), 294–316. DOI: 10.1016/j.recm.2024.05.001
  5. Al-darwesh, M.Y., Ibrahim, S.S., Mohammed, M.A. (2024). A review on plant extract mediated green synthesis of zinc oxide nanoparticles and their biomedical applications. Results in Chemistry, 7, 101368. DOI: 10.1016/j.rechem.2024.101368
  6. Dey, S., Mohanty, D. lochan, Divya, N., Bakshi, V., Mohanty, A., Rath, D., Das, S., Mondal, A., Roy, S., Sabui, R. (2025). A critical review on zinc oxide nanoparticles: Synthesis, properties and biomedical applications. Intelligent Pharmacy, 3(1), 53–70. DOI: 10.1016/j.ipha.2024.08.004
  7. Karam, S.T.; Abdulrahman, A.F. (2022) Green Synthesis and Characterization of ZnO Nanoparticles by Using Thyme Plant Leaf Extract. Photonics, 9, 594. DOI: 10.3390/photonics9080594
  8. Ong, C.B., Ng, L.Y., Mohammad, A.W. (2018). A review of ZnO nanoparticles as solar photocatalysts: Synthesis, mechanisms and applications. Renewable and Sustainable Energy Reviews, 81, 536–551. DOI: 10.1016/j.rser.2017.08.020
  9. Suresh, R., Rajendran, S., Hoang, T.K.A., Vo, D.-V.N., Siddiqui, M.N., Cornejo-Ponce, L. (2021). Recent progress in green and biopolymer based photocatalysts for the abatement of aquatic pollutants. Environmental Research, 199, 111324. DOI: 10.1016/j.envres.2021.111324
  10. Hussain, A., Ali, S., Rizwan, M., Zia ur Rehman, M., Javed, M.R., Imran, M., Chatha, S.A.S., Nazir, R. (2018). Zinc oxide nanoparticles alter the wheat physiological response and reduce the cadmium uptake by plants. Environmental Pollution, 242, 1518–1526. DOI: 10.1016/j.envpol.2018.08.036
  11. Sangeetha, G., Rajeshwari, S., Venckatesh, R. (2011). Green synthesis of zinc oxide nanoparticles by aloe barbadensis miller leaf extract: Structure and optical properties. Materials Research Bulletin, 46 (12), 2560–2566. DOI: 10.1016/j.materresbull.2011.07.046
  12. Singh, S.P., Singh, D. (2010). Biodiesel production through the use of different sources and characterization of oils and their esters as the substitute of diesel: A review. Renewable and Sustainable Energy Reviews, 14, 200–216. DOI: 10.1016/j.rser.2009.07.017
  13. Rasli, N.I., Basri, H., Harun, Z. (2020). Zinc oxide from aloe vera extract: two-level factorial screening of biosynthesis parameters. Heliyon, 6(1), e03156. DOI: 10.1016/j.heliyon.2020.e03156
  14. Pauzi, N., Zain, N.M., Yusof, N.A.A. (2020). Gum arabic as natural stabilizing agent in green synthesis of ZnO nanofluids for antibacterial application. Journal of Environmental Chemical Engineering, 8(3), 103331. DOI: 10.1016/j.jece.2019.103331
  15. Wu, C., Zhang, T., Ji, B., Chou, Y., Du, X. (2024). Green Synthesis of Zinc Oxide Nanoparticles Using Aloe vera Leaf Extract and Evaluation of the Antimicrobial and Antioxidant Properties of the ZnO/Regenerated Cellulose Film. Cellulose, 31(8), 4849–4864. DOI: 10.1007/s10570-024-05914-9
  16. Khaldoune, K., Rafya, M., Louchachha, I., Hasnaoui, A., Benkhalti, F., Fdil, N., Ait Ali, M. (2025). Study on the biosynthesis and antibacterial effect of Zinc Oxide Nanoparticles using aloe vera latex extract. Chemical Papers, 79(8), 5399–5407. DOI: 10.1007/s11696-025-04133-1
  17. Hnawi, S.K., Nayad, A., Aitdads, H., Agdad, A., Afqir, M., Nkhaili, L., El Firdoussi, L., Oueriagli, A., Ait Ali, M. (2021). Investigation of the Structural, Optical, Electrical, and Dielectrical Properties of Aloe Vera Leaf Exudate. Journal of Solar Energy Engineering, 143 (2). DOI: 10.1115/1.4048086
  18. Alavi, M., Nokhodchi, A. (2021). Synthesis and modification of bio-derived antibacterial Ag and ZnO nanoparticles by plants, fungi, and bacteria. Drug Discovery Today, 26(8), 1953–1962. DOI: 10.1016/j.drudis.2021.03.030
  19. Husain, F.M., Hasan, I., Qais, F.A., Khan, R.A., Alam, P., Alsalme, A. (2020). Fabrication of Zinc Oxide-Xanthan Gum Nanocomposite via Green Route: Attenuation of Quorum Sensing Regulated Virulence Functions and Mitigation of Biofilm in Gram-Negative Bacterial Pathogens. Coatings, 10(12), 1190. DOI: 10.3390/coatings10121190
  20. Abu Elella, M.H., Goda, E.S., Gab-Allah, M.A., Hong, S.E., Pandit, B., Lee, S., Gamal, H., Rehman, A. ur, Yoon, K.R. (2021). Xanthan gum-derived materials for applications in environment and eco-friendly materials: A review. Journal of Environmental Chemical Engineering, 9(1), 104702. DOI: 10.1016/j.jece.2020.104702
  21. Ying, S., Guan, Z., Ofoegbu, P.C., Clubb, P., Rico, C., He, F., Hong, J. (2022). Green synthesis of nanoparticles: Current developments and limitations. Environmental Technology & Innovation, 26, 102336. DOI: 10.1016/j.eti.2022.102336
  22. Wu, C., Zhang, T., Ji, B., Chou, Y., Du, X. (2024). Green Synthesis of Zinc Oxide Nanoparticles Using Aloe vera Leaf Extract and Evaluation of the Antimicrobial and Antioxidant Properties of the ZnO/Regenerated Cellulose Film. Cellulose, 31(8), 4849–4864. DOI: 10.1007/s10570-024-05914-9
  23. Chikkanna, M.M., Neelagund, S.E., Rajashekarappa, K.K. (2019). Green synthesis of Zinc oxide nanoparticles (ZnO NPs) and their biological activity. SN Applied Sciences, 1(1), 117. DOI: 10.1007/s42452-018-0095-7
  24. Almoneef, M., Haia H.A., Hendi, A., Aldehish, Merghani, N.M., Alshammari, S.G. (2024) Exploring the multi-faceted potential: Synthesized ZnO nanostructure – Characterization, photocatalysis, and crucial biomedical applications, Heliyon, 10 (12), e32714. DOI: 10.1016/j.heliyon.2024.e32714
  25. Nagaraja, K., Mallika, B., Arunpandian, M., Ravindran, E., Hwan, T., (2025) Green synthesis of gold-decorated BaTiO3-ZnO nanocomposites using Arabic gum polymer for efficient photocatalytic degradation of emerging textile dyes, antimicrobial, and toxicological evaluation, Int. J. Biol. Macromol. 311, 143396. DOI: 10.1016/j.ijbiomac.2025.143396
  26. K.S., J., Jose, J., Li, T., Thomas, M., Shankregowda, A.M., Sreekumaran, S., Kalarikkal, N., Thomas, S. (2020). Application of novel zinc oxide reinforced xanthan gum hybrid system for edible coatings. International Journal of Biological Macromolecules, 151, 806–813. DOI: 10.1016/j.ijbiomac.2020.02.085
  27. Abdelbaky, A.S., Mohamed, A.M.H.A., Sharaky, M., Mohamed, N.A., Diab, Y.M. (2023). Green approach for the synthesis of ZnO nanoparticles using Cymbopogon citratus aqueous leaf extract: characterization and evaluation of their biological activities. Chemical and Biological Technologies in Agriculture, 10(1), 63. DOI: 10.1186/s40538-023-00432-5
  28. Dey, S., Mohanty, D. lochan, Divya, N., Bakshi, V., Mohanty, A., Rath, D., Das, S., Mondal, A., Roy, S., Sabui, R. (2025). A critical review on zinc oxide nanoparticles: Synthesis, properties and biomedical applications. Intelligent Pharmacy, 3(1), 53–70. DOI: 10.1016/j.ipha.2024.08.004
  29. Tabassum, Z., Girdhar, M., Kumar, A., Malik, T., Mohan, A. (2023). ZnO Nanoparticles-Reinforced Chitosan–Xanthan Gum Blend Novel Film with Enhanced Properties and Degradability for Application in Food Packaging. ACS Omega, 8(34), 31318–31332. DOI: 10.1021/acsomega.3c03763
  30. Alhujaily, M., Albukhaty, S., Yusuf, M., Mohammed, M.K.A., Sulaiman, G.M., Al-Karagoly, H., Alyamani, A.A., Albaqami, J., AlMalki, F.A. (2022). Recent Advances in Plant-Mediated Zinc Oxide Nanoparticles with Their Significant Biomedical Properties. Bioengineering, 9(10), 541. DOI: 10.3390/bioengineering9100541
  31. El-Kholy, S.A., Radwan, E.K., El-Naggar, M.E., El-Wakeel, S.T., El-Tantawy El Sayed, I. (2023). Sponge-like zinc oxide nanoparticles loaded xanthan gum/cationic chitosan cryogel: Synthesis, characterization, microbicidal and adsorption of synthetic dye and heavy metal. Journal of Environmental Chemical Engineering, 11(5), 110652. DOI: 10.1016/j.jece.2023.110652
  32. Lal, M., Sharma, P., Singh, L., Ram, C. (2023). Photocatalytic degradation of hazardous Rhodamine B dye using sol-gel mediated ultrasonic hydrothermal synthesized of ZnO nanoparticles. Results in Engineering, 17, 100890. DOI: 10.1016/j.rineng.2023.100890
  33. Bhapkar, A.R., Bhame, S. (2024). A review on ZnO and its modifications for photocatalytic degradation of prominent textile effluents: Synthesis, mechanisms, and future directions. Journal of Environmental Chemical Engineering, 12(3), 112553. DOI: 10.1016/j.jece.2024.112553
  34. Amdeha, E., Salem, M. (2022). Facile Green Synthesis of ZnO Supported on Exfoliated Graphite for Photocatalytic Degradation of Dye under UV and Visible-Light Irradiation. Egyptian Journal of Chemistry, 0(0), 0–0. DOI: 10.21608/ejchem.2022.130532.5931
  35. Liang, L., Cheng, L., Zhang, Y., Wang, Q., Wu, Q., Xue, Y., Meng, X. (2020). Efficiency and mechanisms of rhodamine B degradation in Fenton-like systems based on zero-valent iron. RSC Advances, 10(48), 28509–28515. DOI: 10.1039/D0RA03125A
  36. Purnawan, C., Wahyuningsih, S., Aniza, O.N., Sari, O.P. (2021). Photocatalytic Degradation of Remazol Brilliant Blue R and Remazol Yellow FG using TiO2 doped Cd, Co, Mn. Bulletin of Chemical Reaction Engineering & Catalysis, 16(4), 804–815. DOI: 10.9767/bcrec.16.4.11423.804-815
  37. Arab, C., El Kurdi, R., Patra, D. (2022). Effect of pH on the removal of anionic and cationic dyes using zinc curcumin oxide nanoparticles as adsorbent. Materials Chemistry and Physics, 277, 125504. DOI: 10.1016/j.matchemphys.2021.125504
  38. Kazeminezhad, I., Sadollahkhani, A. (2016). Influence of pH on the photocatalytic activity of ZnO nanoparticles. Journal of Materials Science: Materials in Electronics, 27(5), 4206–4215. DOI: 10.1007/s10854-016-4284-0
  39. El-Taib Heakal, F., Abd-Ellatif, W.R., Tantawy, N.S., Taha, A.A. (2018). Impact of pH and temperature on the electrochemical and semiconducting properties of zinc in alkaline buffer media. RSC Advances, 8(7), 3816–3827. DOI: 10.1039/C7RA12723E
  40. Le, A.T., Samsuddin, N.S.B., Chiam, S.-L., Pung, S.-Y. (2021). Synergistic effect of pH solution and photocorrosion of ZnO particles on the photocatalytic degradation of Rhodamine B. Bulletin of Materials Science, 44(1), 5. DOI: 10.1007/s12034-020-02281-6
  41. Siddique, A.B., Shaheen, M.A., Abbas, A., Zaman, Y., Bratty, M.A., Najmi, A., Hanbashi, A., Mustaqeem, M., Alhazmi, H.A., Rehman, Z. ur, Zoghebi, K., Amin, H.M.A. (2024). Thermodynamic and kinetic insights into azo dyes photocatalytic degradation on biogenically synthesized ZnO nanoparticles and their antibacterial potential. Heliyon, 10(23), e40679. DOI: 10.1016/j.heliyon.2024.e40679
  42. Moghaddasfar, A., Darbandi, M., Li, Z.-A. (2023). Mesoporous cobalt oxide nanoparticles synthesized by a sonochemical method in the presence of a deep eutectic solvent for oxidative sonophotocatalytic decomposition of caffeine. Journal of Water Process Engineering, 54, 104056. DOI: 10.1016/j.jwpe.2023.104056
  43. Groeneveld, I., Kanelli, M., Ariese, F., van Bommel, M.R. (2023). Parameters that affect the photodegradation of dyes and pigments in solution and on substrate – An overview. Dyes and Pigments, 210, 110999. DOI: 10.1016/j.dyepig.2022.110999
  44. Rasheed, H.M., Aroosh, K., Meng, D., Ruan, X., Akhter, M., Cui, X. (2025). A review on modified ZnO to address environmental challenges through photocatalysis: Photodegradation of organic pollutants. Materials Today Energy, 48, 101774. DOI: 10.1016/j.mtener.2024.101774

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